FIGURE 6.
PR-AS390A expression altered growth behavior of MCF-10A cells in three-dimensional culture and expression of PR target genes. PR-A WT, PR-A S390A, or control cells were cultured in a Matrigel-coated chamber slide with medium containing 2% Matrigel. At the indicated time, acini were fixed and stained by antibody against cleaved caspase-3. A and B, acini after 6 (A) and 16 (B) days of culture. C, RANKL expression in MCF-10A cells stably expressing control vector, wild-type PR-A, or S390A mutant PR-A. The cells were fixed after 8 days culture in the three-dimensional Matrigel. D, cyclin D1 expression. See C for details. E, Bcl-2 expression. See C for details.