Figure 1.
RT-PCR amplification of two variants of the histamine H4 receptor messages in RBE4 cells, as well as in a rat spleen sample. Culturing RBE4 cells in regular medium supplemented with 10% calf serum revealed the same two H4 receptor transcripts that are seen in cells after 12 h of starvation (no calf serum supplement). White arrows indicate the transcripts with 1073 bp and 1009 bp CDS, respectively. The contr/RT lane indicates the negative control sample with mRNA omitted from the amplification reaction.