Table 2.
Effect of receptor activation on forskolin-induced cAMP accumulation and [35S]-GTPγS binding
| hH3RWT | hH3RA280V | P | |
|---|---|---|---|
| cAMP accumulation | |||
| Emax (%) | −93.9 ± 2.6 | −57.2 ± 8.4 | 0.003 |
| pEC50 | 8.22 ± 0.35 | 7.75 ± 0.05 | 0.224 |
| [35S]-GTPγS binding | |||
| Emax (% basal) | 129.2 ± 2.6 | 112.6 ± 2.7 | <0.001 |
| pEC50 | 8.50 ± 0.14 | 9.00 ± 0.32 | 0.174 |
| ERK1/2 phosphorylation | |||
| Emax (% basal) | 302 ± 63 | 217 ± 31 | 0.155 |
| pEC50 | 7.94 ± 0.17 | 7.49 ± 0.19 | 0.128 |
Values are means ± SEM from five (cAMP accumulation), eight ([35S]-GTPγS binding) or four (ERK1/2 phosphorylation) experiments. Statistical comparisons were carried out with Student's t-test.