Figure 2.

Incorporation of BrdU in MDA-MB-231 transfected cells. (A)# Error bars represent the mean ± SEM of two independent experiments performed in triplicates. Control, untreated cells; Histamine, cells treated with 10 μmol L⁻¹ of histamine for 18 h; Scrambled siRNA (negative control), cells transfected with scrambled siRNA; H₄R siRNA, cells transfected with specific sequences siRNA designed to knockdown H₄R gene expression; H₄R siRNA+HA, transfected cells with H₄R siRNA and treated with 10 μmol L⁻¹ of histamine for 18 h. (*P < 0.05 vs. Control; P = ns vs. Scrambled siRNA; ANOVA and Dunnett's Multiple Comparison Test). (B) H₄R protein expression in MDA-MB-231 cells was determined by immunocytochemistry. Representative images of H₄R stained cells are shown. x630 original magnification. Scale bar = 20 μm.