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Immunohistochemical analysis of apoptosis and proliferation markers. (A) Representative immunohistochemical images of PCNA, and TUNEL in serial paraffin-embedded tumour specimens; x630 original magnification. Scale bar = 20 μm. (B) Positive stained cells were quantified by counting 10 random fields. Data are presented as a percentage per field. Data are shown as means ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. ANOVA and Newman–Keuls Multiple Comparison Test).
