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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jun 2;63:325–337. doi: 10.1016/j.freeradbiomed.2013.05.040

Figure 2.

Figure 2

Changes in reduced glutathione (GSH) and the oxidized glutathione disulfide (GSSG) measured in VYS, YSF, AF, and E in GD 11 rat conceptuses for controls (solid circles) and following 1mM BSO (open circles) over the 6 hr sampling period. In accord with normal WEC protocols, the time 0 designated on the figure represents the time when cultures are saturated with 95% O2 to ensure continued optimal growth. Control and treated conceptuses are responding to the additional oxygenation over the 6 hr sampling period. The † symbol indicates that control or BSO-treated values have significantly (p< 0.05) increased or decreased at a designated time point relative to the 0 hr media control. The * symbol indicated that BSO treatment produced a significant (p< 0.05) change relative to the media control value at any given time point.