TABLE 1.
Total glutathione and cysteine concentrations found in major tissue and fluid compartments of the rat GD 11 conceptus grown in whole embryo culture. Concentrations were determined using HPLC as described in Methods
| Tissue or Fluid Compartment | Time (hr) | Total Glutathione GSH + 2GSSG (μM) | Total Cysteine cys + 2cySS (μM) | ||
|---|---|---|---|---|---|
| CONTROL | BSO | CONTROL | BSO | ||
| Visceral Yolk Sac | 0 | 5487 ± 493 (12) | 470 ± 57 (12) | ||
| I | 4830 ± 706 (5) | 3219 ± 347a,b (10) | 376 ± 148 (5) | 488 ± 67b (10) | |
| 3 | 6801 ± 1070 (8) | 3111 ± 364a,b (8) | 480 ± 107 (8) | 342 ± 40b (8) | |
| 6 | 8946 ± 1313b (7) | 1073 ± 52a,b (7) | 753 ± 181b (7) | 156 ± 55a,b (8) | |
| Yolk Sac Fluid | 0 | 24 ± 2 (9) | 556 ± 36 (7) | ||
| I | 33 ± 9 (5) | 25 ± 5 (9) | 559 ± 76 (4) | 707 ± 124 (8) | |
| 3 | 63 ± 13b (6) | 19 ± 4a (6) | 1237 ± 343 (6) | 1217 ± 469 (8) | |
| 6 | 65 ± 13b (5) | 25 ± 6a (7) | 454 ± 86 (4) | 1716 ± 559 (7) | |
| Amniotic Fluid | 0 | 11 ± 2 (9) | 42 ± 7 (8) | ||
| I | 13 ± 4 (5) | 10 ± 2 (11) | 44 ± 6 (4) | 120 ± 33a (11) | |
| 3 | 24 ± 6 (6) | 13 ± 2 (8) | 95 ± 16 (5) | 173 ± 70 (8) | |
| 6 | 16 ± 3 (4) | 7 ± 1a (8) | 78 ± 23 (4) | 224 ± 79 (8) | |
| Embryo | 0 | 3186 ± 357 (12) | 491 ± 70 (12) | ||
| I | 2563 ± 383 (5) | 2058 ± 197b) | 391 ± 146 (5) | 606 ± 128 (10) | |
| 3 | 3132 ± 363 (8) | 2099 ± 248a,b (8) | 635 ± 155 (7) | 624 ± 132 (8) | |
| 6 | 4293 ± 481 (7) | 1038 ± 59a,b (8) | 1403 ± 423b (7) | 635 ± 116 (8) | |