Table 2.
A summary of protein concentrations altered in the GD 11 EMB after a 6 hr exposure to 1 mM BSO and as determined by isotope-coded affinity tag (ICAT)-labeling and resolution using mass spectroscopy. Relative changes in protein concentration are quantified based on heavy (treatment)/light (control) isotope ratios. Only proteins showing changes in excess of 15% are shown. The table is also restricted to an analysis of the most abundant EMB proteins and the 10 KEGG pathways with the highest number of altered proteins mapping to that pathway
| Pathway (Mapped from KEGG Database) | % Enrichment | p-value |
|---|---|---|
| A Spliceosome | 2.2 | 0.020 |
| B Glycolysis/Gluconeogenesis | 1.8 | 0.035 |
| C Antigen processing and presentation | 1.8 | 0.039 |
| D Aminoacyl-tRNA biosynthesis | 1.3 | 0.046 |
| E Base excision repair | NS | |
| F Tight junction | NS | |
| G Ribosome | NS | |
| H Purine metabolism | NS | |
| I Cysteine and methionine metabolism | NS | |
| J Selenoamino acid metabolism | NS | |
|
| ||
| Proteins in Significantly Enriched Pathways | GI Accession | H/L |
|
| ||
| Spliceosome | ||
| Acin1 protein [Rattus norvegicus] | 60688451 | 5.17 |
| calcium homeostasis endoplasmic reticulum protein [Rattus norvegicus] | 157823443 | 4.69 |
| PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), isoform CRA_d [Rattus norvegicus] | 149062429 | 3.56 |
| U2af2 protein [Rattus norvegicus] | 59808930 | 3.55 |
| heterogeneous nuclear ribonucleoprotein M isoform b [Rattus norvegicus] | 158186696 | 3.17 |
| Glycolysis/Gluconeogenesis | ||
| glyceraldehyde-3-phosphate dehydrogenase [Rattus norvegicus] [PREDICTED: similar to] | 62653546 | 3.87 |
| dihydrolipoamide dehydrogenase, isoform CRA_a [Rattus norvegicus] | 149051076 | 3.28 |
| Tpi1 protein [Rattus norvegicus] | 38512111 | 2.91 |
| aldolase A, isoform CRA_g [Rattus norvegicus] | 149067838 | 2.59 |
| Antigen Processing and Presentation | ||
| heat shock 70 kDa protein 4 [Rattus norvegicus] | 24025637 | 3.75 |
| protein disulfide isomerase associated 3, isoform CRA_a [Rattus norvegicus] | 149023097 | 2.50 |
| calreticulin, isoform CRA_b [Rattus norvegicus] | 149037838 | 1.97 |
| heat shock protein HSP 90-alpha [Rattus norvegicus] | 28467005 | 1.52 |
| Aminoacyl-tRNA Biosynthesis | ||
| Asparaginyl-tRNA synthetase [Rattus norvegicus] | 68534368 | 5.12 |
| methionyl-tRNA synthetase [Rattus norvegicus] | 189083764 | 3.96 |
| aspartyl-tRNA synthetase, cytoplasmic [Rattus norvegicus] | 16758642 | 3.32 |
|
| ||
| Proteins Most Affected by Treatment from All Pathways | ||
|
| ||
| Protein | GI Accession | H/L |
| peptidyl-prolyl cis-trans isomerase FKBP5 [Rattus norvegicus] [E] | 58865920 | 15.58 |
| heat shock protein 75 kDa, mitochondrial precursor [Rattus norvegicus] | 84781723 | 5.38 |
| Acin1 protein [Rattus norvegicus] [A] | 60688451 | 5.17 |
| Asparaginyl-tRNA synthetase [Rattus norvegicus] [D] | 68534368 | 5.12 |
| APEX [Rattus norvegicus] | 12247526 | 4.76 |
| mitochondrial carrier homolog 2 (C. elegans) (predicted), isoform CRA_b [Rattus norvegicus] | 149022590 | 4.73 |
| calcium homeostasis endoplasmic reticulum protein [Rattus norvegicus] [A] | 157823443 | 4.69 |
| lactate dehydrogenase A-like [Rattus norvegicus] [PREDICTED] | 293344081 | 4.64 |
| Epb4.1l2 protein [Mus musculus] [F] | 27371130 | 4.62 |
| karyopherin (importin) beta 1 [Rattus norvegicus] | 149054034 | 4.48 |
| ribosomal protein S11 [Marmota monax] | 92111517 | 4.45 |
| 60S ribosomal protein L4 [Mus musculus] [G] | 30794450 | 4.42 |
| importin 5 [Rattus norvegicus] [PREDICTED] | 293354006 | 4.41 |
| Ipo4 protein [Mus musculus] | 13097471 | 4.41 |
| phosphoribosylformylglycinamidine synthase [Rattus norvegicus] [H] | 157786806 | 4.40 |
| poly(rC) binding protein 2, isoform CRA_d [Mus musculus] | 148672030 | 4.40 |
| exportin-2 [Rattus norvegicus] | 157820325 | 4.37 |
| b4 integrin interactor [Mus musculus] | 2910995 | 4.36 |
| leucine-rich PPR motif-containing protein, mitochondrial precursor [Rattus norvegicus] | 56605990 | 4.35 |
| eukaryotic translation initiation factor 3, subunit M [Mus musculus] | 123241991 | 4.34 |
| eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked [Mus musculus] | 123270417 | 4.33 |
| Mat2a protein [Mus musculus] [I],[J] | 34849522 | 4.32 |
| T-complex protein 1 subunit eta [Mus musculus] | 238814391 | 4.31 |
| exportin 1, CRM1 homolog (yeast) [Mus musculus] | 123857927 | 4.31 |
| mCG11470 [Mus musculus] | 148686738 | 4.24 |