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. 2013 Mar 25;14:204. doi: 10.1186/1471-2164-14-204

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Copyright © 2013 Wenger and Galliot; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scheme representing the conditions and the reads obtained from Hydra vulgaris for Illumina and 454 RNAseq. A) Left panel: number of intact and regenerating Hydra mixed to extract mRNA used in 454 sequencing. Right panel: length distribution of 454 reads after adapter trimming. B) Left panel: percentages of mRNA taken from Hydra in intact and regenerative conditions, as deduced from their respective number of reads sequenced. Right panel: length distribution of Illumina reads after barcode removal and adapter trimming.