Figure 4.

Evaluation of the completeness of the Hydra-bo transcriptome. A) Construction of the Hydra-meta transcriptome, which contains an unredundant assembly of the Hydra-bo and predicted sequences (pred-CA and pred-RP). B) Saturation analysis of transcripts representation (y-axis) with increasing numbers of sampled reads (x-axis). The Hydra-meta transcriptome was used as a reference. Subsets of randomly sampled Illumina reads were collected and mapped to the reference using Bowtie. The numbers of mapped reads (hits) were counted for each reference sequence.