Table 2.
Immunocytochemistry and Western blot reagents (HRP horseradish peroxidase, DAB diaminobenzidine)
| Primary antibody/sourcea | Dilution | Secondary biotinylated antibody/source | Avidin HRP | Substrate/source |
|---|---|---|---|---|
| Human ICAM1/Santa Cruz | 1:100 | Bovine anti-goat/HRP/ Santa Cruz | Chemiluminescence/Pierce | |
| Human ICAM2/Santa Cruz | 1:100 | Goat anti-rabbit/HRP/Santa Cruz | Chemiluminescence/Pierce | |
| Human VCAM/Santa Cruz | 1:100 | Bovine anti-goat/HRP/Santa Cruz | Chemiluminescence/Pierce | |
| Actin/Santa Cruz | 1:100 | Bovine anti-goat/HRP/Santa Cruz | Chemiluminescence/Pierce | |
| Feline CD4/Tompkins Lab | 1:10 | Mouse link/Biogenix | Biogenix kit | AEC/Biogenix |
| Feline CD8/Tompkins Lab | 1:10 | Mouse link/Biogenix | Biogenix kit | Vector SG/Vector Labs |
| Human CD3/DAKO | 1:100 | Mouse link/Biogenix | Biogenix kit | AEC/Biogenix |
| Feline B cell/Accurate | 1:100 | Link/Biogenix | Biogenix kit | DAB/Biogenix |
| Feline monocyte/Serotec | 1:100 | Mouse link/Biogenix | Biogenix kit | Vector SG/Vector Labs |
a
Santa Cruz Biotechnology, Santa Cruz, Calif.; Tompkins Lab (gift of Dr. Mary Tompkins, Raleigh, N.C.); DAKO, Carpentaria, Calif.; Accurate, Westbury, N.Y.; Serotec, Raleigh, N.C.; Biogenix, San Ramon, Calif.; Pierce Biotechnology, Rockford, Ill.; Vector Labs, Burlingame, Calif.