Fig. 5.

CAPE increases nuclear translocation of XIAP. (A) SK-MEL-28 and (B) B16F0 melanoma cells were treated with 20 µM CAPE for 48 h and nuclear fraction were isolated using nuclear fractionation kit. Represented western blots were immunoblotted with XIAP antibody. Same blots were stripped and re-probed with actin and lamin B to ensure equal protein loading. (C and D) B16F0 cells were treated with dimethyl sulfoxide or 20 µM CAPE, immunostained with XIAP and p-AKT (Ser 473), and actin antibodies and visualized under fluorescence microscope (Olympus Inc). The experiments were repeated three times with similar results obtained.