Figure 7.

Morphology of embryogenic calli and embryos during the six sequential developmental stages of longan SE. The bars in each phenotypic class are indicated at the middle of each image. The morphology of embryogenic cultures friable-embryogenic callus(EC), incomplete compact pro-embryogenic cultures (ICpEC), globular embryos(GE), heart-shaped embryos(HE), torpedo-shaped embryos (TE), and cotyledonary embryos (CE) were observed using an inverted Leica DMIL LED microscope, except for EC(bar=50 μm) and CE(bar=500 μm), the bars of others are 200 μm; EC, ICpEC and GE, were cultured on MS medium supplemented with 1 mg/L, 0.5 mg/L, and 0.1 mg/L 2,4-D, respectively; and the HE, TE and CE were cultured on MS medium.