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RT-PCR analysis of TsAP gene transcript at different stages of T. spiralis. RT-PCR detection of mRNA transcription for the TsAP gene (A) and GAPDH gene (B) at different developmental stages of T. spiralis. M: DNA marker; Lane 1: AW at 3 dpi; Lane 2: ML at 42 dpi; Lane 3: PEL at 19 dpi; Lanes 4: NBL.
