Table 3. Per reader analysis of performance of ZN and LED-based fluorescence microscopy methods, per specimen analysis.
| Overall | Reader 1 | Reader 2 | Reader 3 | Reader 4 | Reader 5 | Reader 6 | |
| Direct ZN Sensitivity | 31.1% (51/164) 24.1–38.8 | 40% (4/10) 12.2–73.8 | 35.9% (23/64) 24.3–48.9 | 26.7% (24/90) 17.9–37.0 | - | - | - |
| Direct ZN Specificity | 99.3% (425/428) 98.0–99.9 | 95.4% (41/43) 84.2–99.4 | 100.0% (125/125) 97.1–100 | 99.6% (259/260) 97.9–100 | - | - | - |
| Direct ZN PPV | 94.4% (51/54) 84.6–98.8 | 66.7% (4/6) 22.3–95.7 | 100.0% (23/23) 85.2–100 | 96.0% (24/25) 79.6–99.9 | - | - | - |
| Direct ZN NPV | 79.0% (425/538) 75.3–82.4 | 87.2% (41/47) 74.3–95.2 | 75.3% (125/166) 68.0–81.7 | 78.6% (259/325) 74.9–83.9 | - | - | - |
| iLED Sensitivity | 40.2% (66/164) 32.7–48.2 | - | - | - | 45.3% (34/75) 33.8–57.2 | 40.9% (9/22) 20.7–63.6 | 34.3% (23/67) 23.2–46.9 |
| iLED Specificity | 93.2% (399/428) 90.4–95.4 | - | - | - | 85.3% (156/183) 79.2–90.0 | 98.2% (54/55) 90.3–100 | 99.5% (189/190) 97.1–100 |
| iLED PPV | 69.5% (66/95) 59.2–78.5 | - | - | - | 55.7% (34/61) 42.4–68.5 | 90.0% (9/10) 55.5–99.7 | 95.8% (23/24) 78.9–99.9 |
| iLED NPV | 80.3% (399/497) 76.3–84.03 | - | - | - | 79.2% (156/197) 72.8–84.6 | 80.6% (54/67) 69.1–89.2 | 81.1% (189/233) 75.4–85.9 |
| Fraen Sensitivity | 42.1% (69/164) 34.4 50.0 | - | - | - | 49.3% (37/75) 37.6–61.1 | 40.9% (9/22) 20.7–63.6 | 34.3% (23/67) 23.2–46.9 |
| Fraen Specificity | 91.6% (392/428) 88.5–94.0 | - | - | - | 82.5% (151/183) 76.2–87.7 | 98.2% (54/55) 90.2–100 | 98.4% (187/190) 95.5–99.7 |
| Fraen PPV | 65.7% (69/105) 55.8–74.7 | - | - | - | 53.6% (37/69) 41.2–65.7 | 90.0% (9/10) 55.5–99.7 | 88.5% (23/26) 69.8–97.6 |
| Fraen NPV | 80.5% (392/487) 76.7–83.9 | - | - | - | 79.9% (151/189) 73.5–85.4 | 80.6% (54/67) 69.1–89.2 | 77.2% (187/231) 75.3–85.8 |
| Lumin Sensitivity | 38.4% (63/164) 0.9–46.3 | - | - | - | 46.7% (35/75) 35.1–58.6 | 36.4% (8/22) 17.2–59.3 | 29.9% (20/67) 19.3–42.3 |
| Lumin Specificity | 91.6% (392/428) 88.5–94.0 | - | - | - | 82.5% (151/183) 76.2–87.7 | 98.2% (54/55) 90.2–100 | 98.4% (187/190) 95.5–99.7 |
| Lumin PPV | 63.6% (63/99) 53.4–73.1 | - | - | - | 52.2% (35/67) 39.7–64.6 | 88.9% (8/9) 51.8–99.7 | 87% (20/23) 66.4–97.2 |
| Lumin NPV | 79.5% (392/493) 75.7–83.0 | - | - | - | 79.1% (151/191) 72.6–84.6 | 79.4% (54/68) 67.9–88.3 | 79.9% (187/234) 74.2–84.9 |
Readers 1–3 read ZN slides, readers 4–6 read LED FM slides (all LED systems for a single specimen were read by the same reader).