Skip to main content
. 2013 Sep 6;8(9):e72262. doi: 10.1371/journal.pone.0072262

Figure 3. Maximum parsimony with bootstrap support for the combined 46 populations.

Figure 3

The tree is based on 54>50% support. Color codes are the same as in Figures 1–2. There are two major monophyletic groupings: one consisting of populations along the Gulf and North Carolina Coasts, the other consisting of all more northern and mountain populations. The mainland Maryland population (MD2) shares a most recent common ancestor with all more northern and mountain populations. Phylogenetic results generally follow latitude except for the mountain populations (NCmt, PA). There are six well supported clades (bootstrap>70) that include two or more populations: Gulf Coast (MS, AL, FL: black), North Carolina Coast (NC: red), North Carolina mountains (NCmt: green), Pocono Mountains (PA: brown), northeast (ME, NS, NL: blue), and northwest (WI2–3, wON, MB, SK: purple). The North Carolina mountain populations (NCmt) split into two clades: one in the Savannah River drainage leading to the Atlantic Ocean (NCmt1–2), the other in the Tennessee River drainage (NCmt3–5) leading to the Gulf of Mexico.