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. 2013 Sep 6;8(9):e72505. doi: 10.1371/journal.pone.0072505

Figure 3. Regulation of monocyte cell cycle by MBL.

Figure 3

(A and B) Cell cycle distribution of U937 cells treated with MBL. FCM analysis of the cell cycle distribution induced by MBL for 24 h at the indicated concentrations (A) and by 8 μg/ml MBL for the indicated times (B). (C–E) The effect of MBL on the mRNA expression of cell cycle regulatory proteins was analyzed by real-time RT-PCR. (C) U937 cells, and (D) monocytes were treated with vehicle, HSA (20 μg/ml) and MBL (20 μg/ml) for 24 h. (E) U937 cells were treated with MBL (8 μg/ml) for different times as indicated. Each bar represents the mean ± S.D. of three independent experiments. * p<0.05, ** p<0.01, *** p <0.001 as compared with the control cultures.