Figure 3.

Dose-dependent anti-mitogenic effect of derivatives 2, 5 and 6 on human melanoma cancer cell lines. Human melanoma cell lines (HTB66 and HTB68) and normal fibroblast (CRL1554) were plated (27x10³ cells/well) into 96 well plates and incubated at 37°C in a CO₂ incubator. Cells were treated with 2 (50–400 μg/mL), 5 (100–800 μg/mL), and 6 (260–400 μg/mL) for 24 h. The cell growth was measured by MTT assay.