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Assessment of apoptosis in human malignant melanoma cells by annexin V-FITC and propidium iodide staining. Untreated and derivative 2-treated (1.3 mg/mL, for 24 h) human malignant melanoma cell lines HTB66 (a) and HTB68 (b) were stained with annexin V-FITC and propidium iodide, then analysed by flow cytometry. B4: percentage of early apoptotic cells, B2: percentage of late apoptotic cells, B1: percentage of necrotic cells and B3: living cells.
