Figure 6.

(a) Schematic of the custom constructed optical system for parallel fluorescence detection in our device. A high power laser diode provides fluorescence excitation on cells through a micro ball lens array. Multicolor fluorescence emission from cells are collected by the same microlens array and imaged on to a high speed CMOS camera through telescope optics. Optical images of different colors are split on the detection image plane by a custom built prism to achieve continuous, simultaneous, multicolor detection of cells passing through all 32 channels. (b) Schematic of the ray tracing shows how multicolor fluorescence signals are collected by a microball lens, color split by a small angle prism, and imaged on to the image plane.