Table 2. FRET efficiency E and distance R between Cy3 labeled SNAP23 and Cy5-labeled Plin2 or GLUT1.
FRET Pair | E (%) | R (Å) |
---|---|---|
SNAP23/Plin2 | 49 ± 1* | 51.3 ± 0.4* |
SNAP23/GLUT1 | 27 ± 1 | 60.4 ± 0.3 |
FRET efficiency E and distance R values were determined as described in the Method section. Values are mean + SE; n = 45-60 cells. (*) indicates p < 0.001 as compared to the SNAP23/GLUT1 FRET pair.