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. 2013 Jul 26;13:60. doi: 10.1186/1472-6750-13-60

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Copyright © 2013 Renz et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bni5 interacts with septins. A. Spin down assay: SDS-PAGE analysis of supernatant and pellet fraction after centrifugation of a sample containing Bni5-SNAP together with septin filaments (low salt (LS); left panel) or together with septin rods (high salt (HS); right panel). Bni5-SNAP co-sedimentation with the septins indicates interaction. B. Validation of the spin down assay with Western blot after SDS-PAGE using an anti-his antibody. 6his tagged Bni5-SNAP is exclusively detected in the pellet with the septins (6his-Cdc12 as control).