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. 2013 Sep 6;8(9):e73817. doi: 10.1371/journal.pone.0073817

Figure 4. p53 binds to a DNA sequence located at -147 of the SALL2 P2 promoter.

Figure 4

A. EMSA assay testing double-stranded oligonucleotide probes containing the putative p53 binding sites located at positions -1879, -147 and +282 of the SALL2 gene (see text for details). The assay includes a -147 probe with two point mutations (-147mµt). The nuclear extract used in this assay was obtained from HCT116 p53 +/+ cells. B. The -147 probe was used in a competition analysis, in the presence of a 50x molar excess of the unlabeled oligonucleotides Cp53, -147mµt (Mut) and -147, as indicated at the top of the figure. This assay used a nuclear extract obtained from HCT116 p53+/+ cells. A and B. The presence of nuclear extract and PAb421 antibody in the binding reactions is indicated at the top of the figures. The migration of free probe and DNA/p53/PAb421 complex is indicated at the right side of the figure, as well as migration of non-specific complexes generated in the presence of nuclear extracts (X).