Table 1.
The primer sequence, product size and annealing temperature of target genes for qRT-PCR
Gene | Primer nucleotide sequence | Product size (bp) | Annealing temperature | Accession no. |
---|---|---|---|---|
β-actin |
5′-ATCGTGGGCCGCCCTAGGCA-3′ (forward) |
243 |
52 |
NM_031144 |
5′-TGGCCTTAGGGTTCAGAGGGG-3′ (reverse) | ||||
Bmp2 |
5′-TAGTGACTTTTGGCCACGACG-3′ (forward) |
81 |
58 |
NM_017178 |
5′-GCTTCCGCTGTTTGTGTTTG-3′ (reverse) | ||||
Bmp4 |
5′-CATGGCTCGCGCCTCCTAGC-3′ (forward) |
184 |
58 |
NM_012827 |
5′- ATTCCGAGCGACGCACTGCC-3′ (reverse) | ||||
Bmp7 |
5′- CAACCTAGTGGAGCACGACAAGGA-3′ (reverse) |
213 |
60 |
NM_001191856 |
5′- AGGTCGGACTCCCTGCCTGAGT-3′ (reverse) | ||||
Bmpr1a |
5′-GCCACCCTGGACACCAGAGC-3′ (forward) |
101 |
60 |
NM_030849 |
5′-GCAGGCTTGCCTTGCGTG-3′ (reverse) | ||||
Bmpr1b |
5′-CACCACGGAGGAAGCCAGC-3′ (forward) |
239 |
60 |
NM_001024259 |
5′-GGCACAGGCCGCTGACAGAC-3′ (reverse) | ||||
Bmpr2 | 5′-GGTGCTGGTCTCACATTG-3′ (forward) |
180 | 58 | NM_080407 |
5′-GAGGCGGACTGAGTGGTG-3′ (reverse) |