Figure 5. Hsp90 expression in Atlantic salmon myoblast cell culture.

A. Atlantic salmon fast skeletal muscle myoblasts at days 4 (i), 6 (ii), 10 (iii) and 14 (iv) of culture. Cells nuclei were stained with SYTOX green and myogenic cells were identified by staining for desmin (blue). B. myogenin expression in myoblast cell culture at days 4, 6, 8, 10, 12 and 14. C. hsp90α1a and hsp90α1b expression in myoblast cell culture at days 2, 4, 6, 10, 12 and 14. To facilitate interpretation of the data, values at day 2 (and 4 for myogenin) were transformed so the mean value = 1. Values represent mean ± SE from 5 different cultures. Different letters indicate significant differences between means (P<0.05).