Table 1. Expression profiles of folding and protein-editing genes in Atlantic salmon myoblasts incubated with amino acid and insulin-like growth factor 1 (IGF1) after 72h of amino acid deprivation.
Time |
12h
|
24h
|
||||||
---|---|---|---|---|---|---|---|---|
Condition | Starvation media | AA | IGF1 | AA+IGF1 | Starvation media | AA | IGF1 | AA+IGF1 |
Gene ID | ||||||||
sgta | nd | nd | nd | nd | 1.0 ± 0.0 | 1.98 ± 0.3 | 0.84 ± 0.1 | 3.36 ± 0.3* |
fkbp4 | nd | nd | nd | nd | 1.0 ± 0.0 | 1.89 ±0.1 | 0.79 ± 2.1 | 2.17 ± 0.1* |
tbcb | nd | nd | nd | nd | 1.0 ± 0.0 | 1.79 ± 0.2 | 0.97 ± 0.1 | 2.66 ± 0.2* |
hsp30 | nd | nd | nd | nd | 1.0 ± 0.0 | 1.54 ± 0.4 | 0.70 ± 0.2 | 2.89 ± 0.1* |
pdia4 | 1.0 ± 0.0 | 2.68 ± 0.9 | 1.06 ± 0.3 | 3.37 ± 0.9* | nd | nd | nd | nd |
pdia6 | 1.0 ± 0.0 | 4.43 ± 1.8* | 1.55 ± 0.9 | 5.67 ± 1.5* | nd | nd | nd | nd |
hsp70 | 1.0 ± 0.0 | 5.53 ± 1.7* | 2.25 ± 0.7 | 4.03 ± 1.0* | 1.0 ± 0.0 | 2.65 ± 1.4 | 5.3 ± 1.1* | 5.02 ± 1.3* |
Values are expressed as mean ± SE from 5 independent Atlantic salmon myoblast cell cultures. Significant differences between treatments for each gene with respect to the cells in “starved media” are indicated with an asterisk (*) (P<0.05).
nd: no statistical difference
Starved media: cells incubated with amino acid free medium
AA: cells incubated with medium containing amino acid
IGF1: cells incubated with medium containing 100ng/ml recombinant Atlantic salmon IGF1
AA+IGF1: cells incubated with a combination of amino acid and IGF1