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. 2013 Sep 6;8(9):e74295. doi: 10.1371/journal.pone.0074295

Table 1. Expression profiles of folding and protein-editing genes in Atlantic salmon myoblasts incubated with amino acid and insulin-like growth factor 1 (IGF1) after 72h of amino acid deprivation.

Time 12h
24h
Condition Starvation media AA IGF1 AA+IGF1 Starvation media AA IGF1 AA+IGF1
Gene ID
sgta nd nd nd nd 1.0 ± 0.0 1.98 ± 0.3 0.84 ± 0.1 3.36 ± 0.3*
fkbp4 nd nd nd nd 1.0 ± 0.0 1.89 ±0.1 0.79 ± 2.1 2.17 ± 0.1*
tbcb nd nd nd nd 1.0 ± 0.0 1.79 ± 0.2 0.97 ± 0.1 2.66 ± 0.2*
hsp30 nd nd nd nd 1.0 ± 0.0 1.54 ± 0.4 0.70 ± 0.2 2.89 ± 0.1*
pdia4 1.0 ± 0.0 2.68 ± 0.9 1.06 ± 0.3 3.37 ± 0.9* nd nd nd nd
pdia6 1.0 ± 0.0 4.43 ± 1.8* 1.55 ± 0.9 5.67 ± 1.5* nd nd nd nd
hsp70 1.0 ± 0.0 5.53 ± 1.7* 2.25 ± 0.7 4.03 ± 1.0* 1.0 ± 0.0 2.65 ± 1.4 5.3 ± 1.1* 5.02 ± 1.3*

Values are expressed as mean ± SE from 5 independent Atlantic salmon myoblast cell cultures. Significant differences between treatments for each gene with respect to the cells in “starved media” are indicated with an asterisk (*) (P<0.05).

nd: no statistical difference

Starved media: cells incubated with amino acid free medium

AA: cells incubated with medium containing amino acid

IGF1: cells incubated with medium containing 100ng/ml recombinant Atlantic salmon IGF1

AA+IGF1: cells incubated with a combination of amino acid and IGF1