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Sarkosyl-insoluble fractions were separated electrophoretically on 4–12% gradient Bis-Tris acrylamide gels and Coomassie stained. Lane 1: Strain P6, Lane 2: Strain PΔ5, Lane 3: Strain PSO1, Lane 4: Strain ¢3. Arrowheads indicate NhhAPMC21 (lane1), NhhABgl,(lane 2), and NhhASO1 (lane 3). Asterisk indicates position of PorA. Apparent MW of ColorPlus markers indicated. See Table 1 for strain and protein descriptions.
