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Pooled sera from mice immunized with OMVs purified from strains over-producing either the wild type NhhAPMC21l (black square), the NhhA lacking variable loops 1&2 (NhhABlg, open triangle), or the NhhA without the four variable regions (NhhASO1, open diamond), and from control mice (open square) immunized with adjuvant alone. Target antigen was recombinant NhhA purified from expression in E. coli.
