Table 1.
Fold changes of gene expression after different treatments on B. anthracis Sterne spores
| Gene |
Control |
SWCNTs-NIR 5 min |
SWCNTs-NIR 25 min |
|||
|---|---|---|---|---|---|---|
| ΔCt* | ΔCt | Fold change** | ΔCt | Fold change | ||
| Germination related genes |
gerLA |
20.50 ± 0.84 |
20.31 ± 0.96 |
1.14 |
19.45 ± 0.71 |
2.07 |
|
gerLB |
22.61 ± 1.04 |
22.60 ± 0.99 |
1.01 |
21.54 ± 0.42 |
2.11 |
|
|
gerYA |
19.80 ± 0.78 |
20.58 ± 0.27 |
0.58 |
20.83 ± 0.30 |
0.49 |
|
|
gerN |
21.74 ± 1.32 |
19.51 ± 0.91 |
4.68 |
21.31 ± 0.33 |
1.35 |
|
|
gerHC |
20.84 ± 1.14 |
20.64 ± 0.54 |
1.14 |
20.61 ± 1.01 |
1.17 |
|
|
gerKB |
21.27 ± 1.12 |
20.50 ± 0.92 |
1.71 |
23.17 ± 3.60 |
0.26 |
|
| Regulatory genes |
atxA |
22.48 ± 0.39 |
23.36 ± 0.47 |
0.54 |
23.64 ± 0.66 |
0.45 |
|
pagR |
21.85 ± 0.18 |
23.15 ± 0.46 |
0.41 |
22.61 ± 0.82 |
0.59 |
|
| GBAA1941 |
22.36 ± 0.30 |
20.75 ± 0.80 |
3.05 |
21.39 ± 0.14 |
1.96 |
|
| Virulence related genes |
cya |
21.91 ± 0.15 |
23.02 ± 0.13 |
0.46 |
23.79 ± 0.41 |
0.27 |
|
lef |
22.74 ± 0.33 |
23.38 ± 0.14 |
0.64 |
24.74 ± 0.18 |
0.25 |
|
|
pagA |
21.45 ± 0.52 |
22.35 ± 0.24 |
0.54 |
23.07 ± 0.34 |
0.33 |
|
| GBAA5564 |
22.59 ± 0.34 |
22.14 ± 0.17 |
1.37 |
22.75 ± 0.22 |
0.89 |
|
| GBAA0797 |
22.62 ± 0.13 |
22.83 ± 0.20 |
0.86 |
22.63 ± 0.30 |
0.99 |
|
|
purF |
23.51 ± 0.74 |
24.92 ± 0.19 |
0.37 |
23.23 ± 0.23 |
1.21 |
|
| srtB | 23.78 ± 0.06 | 26.13 ± 0.89 | 0.2 | 24.30 ± 0.10 | 0.7 | |
*The ΔCt value (Ct, threshold cycle) of each tested gene was a relative expression level compared to the value of housekeeping gene 16S rRNA. The ΔCt values are expressed as the average of three replicates ± standard deviation.
**The fold change is determined as 2-ΔΔCt.