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. 2013 Sep 6;8(9):e74928. doi: 10.1371/journal.pone.0074928

Figure 2. 53BP1 sensitizes breast cancer cells to 5-Fu in vitro.

Figure 2

(A) The transfection efficiency was measured by western blot analysis (left) and qRT-PCR (right). Results are shown for one of the three independent experiments performed. **p<0.01, ***p<0.001. (B) MDA-MB-231 control and 53BP1 overexpressing cells were treated with 5-Fu for 24 h (left) or 48 h (right). The effect of 53BP1 on 5-Fu sensitivity was measured by MTT assay. (C) MCF-7 control and 53BP1 knockdown cells were treated with 5-Fu for 24 h (left) or 48 h (right). The effect of 53BP1 on 5-Fu sensitivity was measured by MTT assay. The relative cell viability percentage is expressed as (absorbance of treated wells/absorbance of untreated wells).