Table 2. Effect of 5-Fu on cell viability assessed by the MTT method (uM).

	IC50 (mean ± sd)
	24 h	48 h
MCF-7 Control NC	18.44±3.81	8.07±1.35
MCF-7 Control siRNA TS	16.72±2.93	10.54±2.74
MCF-7 Control siRNA DPYD	16.76±1.59	8.64±1.62
MCF-7 shRNA 1 NC	48.11±2.93	19.3±1.24
MCF-7 shRNA 1 siRNA TS	24.75±1.07	11.04±0.97
MCF-7 shRNA 1 siRNA DPYD	18.92±1.53	14.46±0.75

IC50 is the half maximal inhibitory concentration. The IC50 ± sd were calculated based on the results of three independent experiments.