Table 4.
Immunophenotype precision
| BM MSCs Markers | CV% Inter-experiment |
|---|---|
| CD90 FITC |
6.22 |
| CD73 PE |
0.95 |
| CD105 APC |
1.86 |
| CD45-34-14 FITC |
3.13 |
| HLADR PE |
4.46 |
| CD19 APC |
3.84 |
|
iDCs Markers |
CV% Inter-experiment |
| CD83 FITC |
2.2 |
| CD40 PE |
10 |
| HLADR PERCPCY5 |
4.6 |
| CD11c APC |
2.7 |
| CD80 FITC |
8.4 |
| CD86 PE |
7.1 |
|
mDCs Markers |
CV% Inter-experiment |
| CD83 FITC |
3.6 |
| CD40 PE |
7.9 |
| HLADR PERCPCY5 |
6.3 |
| CD11c APC |
5.4 |
| CD80 FITC |
5.1 |
| CD86 PE |
3.9 |
|
CTL-LS Markers |
CV% Inter-experiment |
| CD3 FITC |
9.7 |
| CD8 PE |
10 |
| CD4 APC |
8.1 |
| CD45 PERCP |
8.1 |
| CD56 PE |
7.0 |
| CD19 APC |
8.0 |
|
CTL-E Markers |
CV% Inter-experiment |
| CD45RA FITC |
9.4 |
| CD45RO PE |
10 |
| CD4 APC |
3.7 |
| CD3 PERCP |
6.9 |
| CD8 APC | 6.4 |
The immunophenotype test was performed three times under the same operating conditions by the same operator on our CTPs. The inter experiment CV% was calculated considering the mean and SD of the mean fluorescence intensity (MFI) of each cytofluorimetric marker.
The acceptance criteria was an inter-experiment CV% ≤ 10%.
Abbreviations:CTL LS Cytotoxic T Lymphocyte subpopulations, CTL E Cytotoxic T Lymphocyte effectors.