Table 5.
Primers (RT-PCR and qPCR)
| Gene/variant Symbol | Forward Primer | Reverse primer | Size (bp) |
|---|---|---|---|
| CEACAM1-4L |
AAGACGATCATAGTCACTGAGCT |
GGAGACTGAGGGTTTGTGCT |
483 |
| CEACAM1-4S |
AAGACGATCATAGTCACTGAGCT |
ATTGGAGTGGTCCTGAGCTG |
454 |
| CEACAM1-3L |
TCATAGTCACTGATAATGCTCTACC |
GGAGACTGAGGGTTTGTGCT |
188 |
| CEACAM1-3S |
TCATAGTCACTGATAATGCTCTACC |
ATTGGAGTGGTCCTGAGCTG |
159 |
| CEACAM1-4C1 |
AAGACGATCATAGTCACTGAGCT |
TTGCACACCATTGACAGAGT |
369 |
| CEACAM1-3 |
CAGTGACCCAGTCACCTTGA |
TGGACTTGTTTGTGCCTGTTG |
403 |
| CEACAM1-3C2 |
CAAGACGATCATAGTCACTGAGTC |
AGAGGGACATATAGGAAGGGGT |
210 |
| CEACAM5 |
AGGCCAATAACTCAGCCAGT |
GGGTTTGGAGTTGTTGCTGG |
104 |
| CEACAM6 |
TCAGCCACTGGCCTCAATAG |
TCTGGTCCAATCTGCCAGTC |
177 |
| HPRT1 |
GACCAGTCAACAGGGGACAT |
AACACTTCGTGGGGTCCTTTTC |
195 |
| PPIB |
ATGTAGGCCGGGTGATCTTT |
TGAAGTTCTCATCGGGGAAG |
219 |
| IFNβ-1a | TGCTCTCCTGTTGTGCTTCT | CCACAGGAGCTTCTGACACT | 103 |
Structure of forward and reverse primers (5′-3′) of all target genes and isoforms and the expected size of their respective amplified PCR-products.