Table 1.
Oligonucleotide sequences.
| PCR product | Size (bp) | Restriction site | Oligonucleotide sequence (5′ - 3′) |
|---|---|---|---|
|
M. hyopneumoniae strain 232 oriC- i |
2130 |
NcoI |
F - AGGCCATGGTTGTTAATTATTGCTTGAAATTC |
| |
|
SpeI |
R - AGTACTAGTAAACTTTATAGGAAAGTTCG |
|
M. hyopneumoniae strain 232 oriC- ii |
1823 |
NcoI |
F - GATGCCATGGTTGGTTGATAAGTTCGCTTC |
| |
|
SpeI |
R - GTAGACTAGTCATTCTTTCAATTTGTCATTC |
|
M. hyopneumoniae strain 232 oriC- iii |
859 |
NcoI |
F - GATGCCATGGTTGGTTGATAAGTTCGCTTC |
| |
|
SpeI |
R - GCTCACTAGTGACAATCAGTTTAAAAATTCAC |
|
M. hyopneumoniae strain 232 oriC- iii |
859 |
AatII |
F - GCAGGACGTCTTGGTTGATAAGTTCGCTCC |
| |
|
NcoI |
R - GACGCCATGGGACAATCAGTTTAAAAATTCAC |
|
M. hyopneumoniae strain 232 oriC- iv |
422 |
NcoI |
F - GCATCCATGGATATTGTGCATGCCCGCGGATAT |
| |
|
SpeI |
R - GTAGACTAGTCATTCTTTCAATTTGTCATTC |
|
M. hyopneumoniae strain 232 oriC- v |
280 |
AatII |
F - GCAGGACGTCTTGGTTGATAAGTTCGCTCC |
| |
|
NcoI |
R - CGTACCATGGCCTAATTTGCTGTGTTCTAA |
|
tetM + spiralin promoter from plasmid pSRT2 |
2320 |
PstI |
F - TAACTGCAGCAAAAGCTTGCATGCCTGCA |
| |
|
SpeI |
R - GTCACTAGTCAGTTCAGATCTTTATATAAC |
|
tetM from plasmid pIVT |
2109 |
SpeI |
F - ATCTACTAGTCATGTGATTCTAAAGTATCC |
| |
|
PstI |
R - GATCTGCAGCTTATTTAACAAGAAACC |
|
tetM (without promoter) from plasmid pSRT2 |
1963 |
BamHI/PstI |
F - GAACTGCAGGATCCATGGAGGAAAATCACATGA |
| |
|
SalI |
R - CCCGGTCGACTTATATAACAACTTAAATTAC |
|
M. hyopneumoniae strain 232 P97 promoter |
619 |
BamHI |
F - GAATGGATCCCCAACAATTCCGGCAGTC |
| |
|
SpeI |
R - GCTAACTAGTACGGGGATTTAAAACAGAAAC |
|
M. hyopneumoniae strain 232 ldh promoter |
421 |
SpeI |
F - GGCTAACTAGTATAGAATTTTGCAATTAAAG |
| |
|
BamHI |
R - CTTAGGATCCATTTATACTCGTATTTGTTAT |
|
M. hyorhinis oriC |
1479 |
NcoI |
F - GACTCCATGGTCGGAATCGAGATTCCTAATT |
| |
|
SpeI |
R - GCCTACTAGTCTACACCTTCGATTTCTCTAA |
|
M. pulmonis oriC |
1909 |
NcoI |
F - ATTAGCCATGGCACTCTGGTCAGCGCTAGATC |
| |
|
NheI |
R - TCGCGCTAGCCTAACTTGAAAATAAGCTCC |
|
M. conjunctivae oriC |
2307 |
NcoI |
F - GATACCATGGTATGCCTTTGGTCTAGGATG |
| |
|
XbaI |
R - CGATGTCTAGACCCTTGAACAAATATACCA |
|
M. ovipneumoniae oriC |
2102 |
NcoI |
F – AGTCCATGGTCAATTATTGCTTGGAATTC |
| |
|
SpeI |
R - CGTACTAGTAAACTTTATAAGAAAGTTCGCT |
|
bla gene (DIG-labelled probe) |
860 |
N/A |
F – CCAATGCTTAATCAGTGAGG |
| |
|
|
R - GTATGAGTATTCAACATTTCCG |
|
tetM (DIG-labelled probe) |
406 |
N/A |
F – GTGGACAAAGGTACAACGAG |
| R - CGGTAAAGTTCGTCACACAC |
Oligonucleotide sequences are shown along with the size of the PCR product generated and the template DNA used. Restriction enzyme sites (where applicable) that were added to the 5′ end of the oligonucleotide are shown in italics. Forward (F) and reverse (R) oligonucleotides are shown.