Table 2.
Effect of electroporation voltage on transformation frequency.
| Electroporation voltage | Average transformation frequency (transformants/CFU) [SE] |
|---|---|
| 2.5 kV |
5.4 × 10-6 [1.2 × 10-6] |
| 2.0 kV |
4.2 × 10-7 [3.9 × 10-7] |
| 1.5 kV |
0 |
| 1.0 kV |
0 |
| 0 kV | 0 |
The effect of altering the electroporation voltage on transformation frequency was examined. M. hyopneumoniae strain 232 was transformed with pMHO-2 and the average transformation frequencies (with standard error) of three separate transformations are shown.