Figure 2.

FFT Analysis of chronological skin ageing. Representative images of 3mth, 8mth, 12mth and 20mth C57Bl6 mouse skin stained with picrosirius and viewed under cross-polar optics (original magnification 90X; scale bar = 20 μm). The graph shows the collagen orientation index derived from the FFT, the bar equates to the mean and hair lines are standard error of the mean (S.E.M.). Statistical comparisons were achieved by comparing values via one way ANOVA with post-hoc Dunnett’s test compared to 3mth animals, n > 3 animals per group.