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. 2013 Jul 12;10:231. doi: 10.1186/1743-422X-10-231

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Copyright ©2013 Ali et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Southern blot detection of begomoviruses in inoculated N. benthamiana plants. Blots were probed for the presence of CLCuBuV (A), CLCuKoV (B), PedLCuV (C) or ToLCNDV (D). For each blot DNA samples were extracted from inoculated transgenic plant harbouring the P1C (lanes 1 and 2) or P1D (lanes 3 and 4) constructs or inoculated non-transgenic plants (lanes 6 and 7; note DNA from only a single plant was used in panel A). DNA extracted from a non-inoculated transgenic plant was run in lane 5. Approx. 10 μg of total DNA was loaded in each case. The positions of viral single-stranded (ss), super-coiled (sc), linear (lin) and open-circular (oc) DNA forms are indicated.