Table 4.
Infectivity of ToLCNDV in transgenic N. benthamiana plants harbouring amiRNA
| Plant |
Infectivity |
Diagnostics |
|||||
|---|---|---|---|---|---|---|---|
|
(plants symptomatic/plants inoculated) | |||||||
|
Exp. 1 |
Exp.2 |
PCR£ |
Southern$ |
||||
| 15dpi | 28dpi | 15dpi | 28dpi | Exp. 1 | Exp.2 | ||
|
N. b |
10/10 |
10/10 |
9/10 |
10/10 |
10/10 |
10/10 |
++++ |
| P1C |
9/10 |
10/10 |
7/10 |
10/10 |
10/10 |
10/10 |
+++ |
| P1D |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
++++ |
|
N. b* |
0/10 |
0/10 |
0/10 |
0/10 |
0/10 |
0/10 |
− |
| N. b# | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | − |
* Non-transgenic N. benthamiana plants inoculated with Agrobacterium cultures harbouring pGreen0029.
# Non-inoculated plants.
£ ToLCNDV was detected in nucleic acids extracted from plants by PCR using primers ToLCNDVV2F and ToLCNDVV2R (Table 5).
$ Southern hybridization results are given as strong hybridization (++++), through weak (+) hybridization to no hybridization detected (−).