Table 5.
List of oligonucleotides primers used in the study
| Primer | Sequence (5′-3′) | Virus/ Construct | Size of amplicon (bp) | Amplicon |
|---|---|---|---|---|
| BAF |
ACGCGTGCCGTGTTGCTGCCCCATTGTCC |
begomovirus |
~2800 |
genome |
| BAR |
ACGCGTATGGGCTGYCGAAGTTSASACG |
|
|
|
| BV2F |
ATGTGGGATCCACTGTTAAATGAG |
CLCuBuV |
356 |
V2 gene |
| BV2R |
TAGGAACATCTGGACTTCTGTAC |
|
|
|
| CLCKV2F |
GTCGACAAGTATGCGTTTGAAAAATGTG |
CLCuKoV |
370 |
V2 gene |
| CLCKV2R |
GGATCCACCTTCACATCCTCTAGGAAC |
|
|
|
| IRVF |
CGTGGAATTCATGTGGGATCCACTGTT |
CLCuBuV |
348 |
V2 gene |
| IRVR |
TTCGTCGACGAACATCTGGACTTCTGTA |
|
|
|
| PedLCV2F |
ATGTGGGATCCGTTATTGAAC |
PedLCuV |
357 |
V2 gene |
| PedLCV2F |
CTAGGAACATCTGGACTTCTG |
|
|
|
| TLCNDV2F |
GTCGACAAACATGTGGGATCCATTATTGC |
ToLCNDV |
361 |
AV2 gene |
| TLCNDV2R |
CCCGGGCTTCTATACATTCTGTAC |
|
|
|
| P1CF |
GACAGTGGTCCCAAAGATGGA |
P1C |
273 |
expression cassette* |
| P1CR |
ATCGGGAATAATGGTCCTAA |
|
|
|
| P1DF |
GACAGTGGTCCCAAAGATGGA |
P1D |
275 |
expression cassette* |
| P1DR |
TAGCCCATATGCCCGGATTG |
|
|
|
| 35SF |
GACAGTGGTCCCAAAGATGGA |
pJIT163 |
150 |
CaMV 35S promoter |
| 35SR |
CAGTGGAGATATCACATCAATCCA |
|
|
|
| TLCNDBF |
GTCCCGGGTATGTCACGTATCCATCA |
ToLCNDV DNA B |
550 |
NSP gene |
| TLCNDBR | TATCGATTTATCCAATGTAATTAAGAAT |
* These primer pairs amplify from the 3′ end of the CaMV 35S promoter (P1CF and P1DF) to the 3′ end of the amiRNA construct (primers P1CR and P1DR being the reverse complement of the sequence of the amiRNA backbone [amiRNA* sequence]).