. 2013 Aug 29;13:195. doi: 10.1186/1471-2180-13-195

Table 1.

Genotype of bacterial strains, plasmids and ICE R391 mutants used

Strain	Genotype	Source
AB1157	F^-, thr-1, araC14, leuB6, ∆(gpt-proA)62, lacY1, tsx-33, qsr’-0, glnV44, galK2, λ-, Rac-0, hisG4, rfbC1, mgl-51, rpoS396, rpsL31 (Str^R), kdgK51, xylA5, mtl-1, argE3, thi-1	E. coli genetic stock centre (CGSC), Yale University, New Haven, Connecticut, USA
TOP10	F^-, mcrA0, ∆(mrr-hsdRMS-mcrBC), φ80dlacZ58(M15), ∆lacX74, recA1, araD139, ∆(araA-leu)7697, galU^-, galK0, rpsL^- (Str^R), endA1, nupG^-	Bio-Sciences, Dun Laoghaire, Dublin, Ireland
P125109	S. Enteritidis PT4 wild type (NCTC 13349), Nal^R	National Collection of Type Cultures (NCTC), Salisbury, UK
Plasmid	Genotype	Source
pBAD33-orf43	Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43	Armshaw and Pembroke, 2013 [8]
pBAD33-orf43[SM12]	Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43 containing mutation converting two leucines to prolines at a.a. position 47 and 48.	This study
pBAD33-orf43[SM56]	Cm^R, p15A ori, P_BAD L-arabinose inducible, orf43 containing mutation converting glutamine at position 115 to asparagine.	This study
pKOBEG	T_s, P_BAD-gam-bet-exo cat (Cm^R)	Dr. P. Latour-Lambert, Institut Pasteur, 25 rue du Dr Roux, Paris, France
pUC18	Am^R template for deletion mutant construction	Sigma-Aldrich, Arklow, Wicklow, Ireland
pcDNA3.1⁽⁺⁾	Ze^R template for deletion mutant construction	Invitrogen, Bio-Sciences, Dun Laoghaire, Dublin, Ireland
ICE	Genotype	Source
R391	Km^R, Hg^R	Dr R.W. Hedges, Royal Postgraduate Medical School, London, UK
R391 Mutant	Genotype	Source
AB1157 R391 ∆14 (∆orf43)	ICE R391 orf43 deletion strain, Am^R, UV^-, tra^-	Armshaw and Pembroke, 2013 [8]
AB1157 R391 ∆26 (∆orfs90/91)	ICE R391 orfs90/91 deletion strain, Am^R, UV^-, tra^-	Armshaw and Pembroke, 2013 [8]
AB1157 R391 ∆11 (∆orfs40/41)	ICE R391 orfs40/41 deletion strain, Am^R, tra^-	Armshaw and Pembroke, 2013 [8]
AB1157 R391 ∆25_Am^R∆14_Ze^R	ICE R391 orf90 – orf94 and orf43 deletion strain, Am^R, Ze^R, UV^-, tra^-	This study
AB1157 R391 KOA	ICE R391 orf32 - orf42 (29575 bp – 41491 bp) deletion strain, Am^R, tra^-	This study
AB1157 R391 KOB	ICE R391 orf32 - orf42 (29575 bp – 41527 bp) deletion strain, Am^R, UV^-, tra^-	This study
AB1157 R391 KOC	ICE R391 orf32 - orf42 (29575 bp – 41491 bp) and orfs90/91 deletion strain, Am^R, Ze^R, UV^-, tra^-	This study

Str^R is streptomycin resistant; Cm^R is chloramphenicol resistant; Km^R is kanamycin resistant; Hg^R is mercury resistant; Ze^R is zeocin resistant; T_s is temperature sensitive; Nal^R is nalidixic acid resistant and Am^R is ampicillin resistant. UV^- is UV-inducible cell-sensitising function abolished; tra^- is conjugative transfer deficient mutant.