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. 2013 Jan 29;2013:907821. doi: 10.1155/2013/907821

Figure 7.

Figure 7

Sections stained for the demonstration of NK-1R (a)–(c). In (d, e), double-staining for SP (d) and NK-1R (e) is shown. In (f, g), double staining for NK-1R (f) and S100beta (g) is shown. Specimens are from gastrocnemius muscle, exercised side, 1-week group (a, d, e), gastrocnemius muscle, nonexercised side, 6-week group (b, f, g), and soleus muscle, exercised side, 6-week group (c). In (a), strong NK-1R immunoreactions are seen in nerve fibers (arrows) and weaker immunoreactions in inflammatory cells (arrowheads). In (b, c), immunoreactive nerve profile reactions are seen (arrows). In (d, e) it is obvious that there is only partial colocalisation between SP- and NK-1R immunoreactions in the nerve fibers (arrows). In (f), coalesced NK-1R immunoreactive profiles are seen. These are related to a markedly S100beta-immunoreactive cell (arrowhead at nucleus). Note that this nucleus exhibits a pink colour reaction; other nuclei in (g) show the characteristic bluish DAPI reaction. M= muscle fiber (f, g). Bars = 25 μm.