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. 2013 Aug 5;14(1):82. doi: 10.1186/1465-9921-14-82

Figure 5.

Figure 5

PCN induces the expression of mucins in polarized NHBE and 16HBE airways epithelial cells. (A-D) Induction of MUC5AC mucin expression in polarized NHBE (A) and 16HBE (C) cells cultured at the air-liquid interface by PCN (12.5 μg/ml). Epithelial cells exposed to IL-13 (1 μg/ml) or sterile water (12.5 μl/ml) were used as positive or negative control for MUC5AC induction, respectively. MUC5AC induction by PCN (B) and IL-13 (D) was quantified by the ratio of MUC5AC fluorescence signal (green) divided by the total fluorescence signal of DAPI stained nuclei (blue) using the AxioVision Rel. 4.8 software.