Figure 1.

Agarose gel electrophoresis of amplified ITS-2 and cox-1; evaluation of the specificity of our PCR method and the method previously described [23]: 1. adult Fasciola hepatica; 2. adult Fascioloides magna; 3. Lymnaea palustris experimentally infected with F. hepatica; 4. L. palustris experimentally infected with F. magna; 5. Galba truncatula naturally infected with Haplometra cylindracea. The first five (1–5) PCR products (ITS-2) were obtained according to our described ITS-2 gene amplification method (from the left side); five others (cox-1) were obtained according to the previously used PCR protocol with primers for mitochondrial cytochromoxidase 1 [23].