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Results of the polymerase chain reaction (PCR) amplification of a) E. histolytica, b) E. dispar and c) E. moshkovskii with different volume of cysts. Lane 1, molecular weight marker; Lanes 2–6, amplification with 10, 5, 2.5, 1.25 and 0.625 cysts respectively (2 fold serial dilutions were performed); Lane 7, negative control.
