Figure 3.

Experimental Manipulations of Distance-Based Discrimination

(A) Maximum intensity projection of 117 confocal sections (1.5 μm) through the central brain of a fly carrying NP3062-GAL4:UAS-mCD8-GFP transgenes.

(B) Single confocal sections from anterior (B₁) to posterior (B₃) of the antennal lobe region indicated in (A).

(C) Absolute decision bias scores of flies carrying NP3062-GAL4:UAS-shi^ts1 transgenes as functions of Euclidean or cosine distances between ePN activity vectors (mean ± SEM, n = 30–40 flies per data point). For each odor pair, colored arrows indicate the behavioral change caused by shifting flies from the permissive to the restrictive temperature. The distance-discrimination functions of WT flies, obtained in Figure 2D, are reproduced for reference. The decision bias scores of NP3062-GAL4:UAS-shi^ts1 flies differ significantly between the permissive and restrictive temperatures, as predicted from the reduction in ePN distances (p = 0.0109 and 0.0411 for Euclidean and cosine distance, respectively; F test).

(D) Maximum intensity projection of 111 confocal sections (1.5 μm) through the central brain of a fly carrying NP1579-GAL4:UAS-mCD8-GFP transgenes.

(E) Single confocal sections from anterior (E₁) to posterior (E₃) of the antennal lobe region labeled in (D).

(F) Absolute decision bias scores of flies carrying NP1579-GAL4:UAS-shi^ts1 transgenes as functions of Euclidean or cosine distances between ePN activity vectors (mean ± SEM, n = 40 flies per data point). For each odor pair, colored arrows indicate the behavioral change caused by shifting flies from the permissive to the restrictive temperature. The distance-discrimination functions obtained in Figure 2D are reproduced for reference. The decision bias scores of NP1579-GAL4:UAS-shi^ts1 flies differ significantly between the permissive and restrictive temperatures, as predicted from the reduction in ePN distances (p < 0.0001 for Euclidean and cosine distances; F test).

See also Table S3 and Figure S3.