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. 2013 Aug 15;6:27. doi: 10.1186/1756-8935-6-27

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Copyright © 2013 Bhaskara et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SMARCA5 associates with candidate replication origins during S-phase. Serum-starved (SS, 0 h) NIH3T3 cells were released into S-phase in the presence of DMSO or 3 μM 898. SMARCA5 occupancy at α-globin (early replicating origin), pancreatic amylase (mid-late replicating) and β-globin (late replicating) in synchronous NIH3T3 cells were determined using chromatin immunoprecipitation (ChIP) assays. Average fold enrichment for SMARCA5 relative to input from two independent experiments is shown.