Table 1.
IPTgene family in Brassica rapa , along with their molecular details and relevant genomic information
|
Gene namea |
Locusb |
Chrc |
Sub- genomed |
ORF lengthe(bp) |
Deduced polypeptidef |
TargetPg |
Glycosylation Sitesh |
||
|---|---|---|---|---|---|---|---|---|---|
| Length (aa) | MW (kDa) | PI | |||||||
|
BrIPT1-1 |
Bra004037 |
A07 |
MF2 |
1002 |
333 |
37.8 |
8.91 |
C/3 |
0 |
|
BrIPT1-2 |
Bra033933 |
A02 |
MF1 |
1095 |
364 |
41.6 |
8.64 |
C/1 |
0 |
|
BrIPT2 |
Bra034366 |
A04 |
MF1 |
1392 |
463 |
52.4 |
5.89 |
— |
1 |
|
BrIPT3-1 |
Bra040431 |
A04 |
MF1 |
1005 |
334 |
37.3 |
8.11 |
C/4 |
2 |
|
BrIPT3-2 |
Bra007728 |
A09 |
LF |
999 |
332 |
37.6 |
8.4 |
C/5 |
1 |
|
BrIPT5-1 |
Bra002204 |
A10 |
LF |
1005 |
334 |
37.9 |
6.35 |
— |
1 |
|
BrIPT5-2 |
Bra023701 |
A02 |
MF2 |
996 |
331 |
37.6 |
5.75 |
— |
1 |
|
BrIPT7-1 |
Bra014968 |
A07 |
LF |
999 |
332 |
37.1 |
8.68 |
C/5 |
1 |
|
BrIPT7-2 |
Bra028326 |
A01 |
MF1 |
906 |
301 |
33.6 |
8.71 |
C/3 |
1 |
|
BrIPT8-1 |
Bra037537 |
A01 |
MF1 |
975 |
324 |
36.9 |
8.5 |
C/2 |
0 |
|
BrIPT8-2 |
Bra001737 |
A03 |
MF2 |
984 |
327 |
37.0 |
9.26 |
C/4 |
1 |
|
BrIPT9-1 |
Bra006535 |
A03 |
MF1 |
1392 |
463 |
52.2 |
7.51 |
C/5 |
3 |
| BrIPT9-2 | Bra020081 | A02 | MF2 | 1395 | 464 | 52.2 | 7.96 | C/5 | 3 |
aGene Names given to BrIPTs in this work.
bLocus represented by the Brassica rapa genome database.
cChromosomal localization of the BrIPTs and BrCKXs, sca represents scaffold.
dThree sub-genomes of Brassica rapa genome.
eLength of open reading frame in base pairs.
fLength (number of amino acids), molecular weight (kDa), and isoelectric point (pI) of the deduced polypeptide.
gLocalization predicted with TargetP. M, mitochondria; C, chloroplast; S, secretory pathways; “—”, unknown location. (1 to 5), highest to lowest possibility.
hGlycosylation sites are predicted with NetNGly.