Table 3.
Plasmids used in this study
| Name | Relevant genotype | Source/reference |
|---|---|---|
| pFL451 |
AOX1p-prk (Spinach)-AOX1t (pHIL2-D2 HIS4 Amp centromeric) |
Brandes et al.[9]. |
| pCR®-Blunt II-TOPO |
Bla |
Life Technologies Europe BV |
| pTEF424_TEF |
TRP1 2μ bla |
Mumberg et al.[28]. |
| pSH47 |
URA3 CEN6 ARS4 GAL1p-cre-CYC1tbla |
Güldener et al. [29]. |
| pUD0E46 |
TRP1 2μ GAL1p-prk-CYC1tbla |
This study. |
| pPCR-Script |
Bla |
Life Technologies Europe BV. |
| pGPD_426 |
URA3 2μ bla |
Mumberg et al.[28]. |
| pRS416 |
URA3 CEN6 ARS4 bla |
Mumberg et al.[28]. |
| pBTWW002 |
URA3 2μ TDH3p-cbbM-CYC1tbla |
This study. |
| pUDC098 |
URA3 CEN6 ARS4 TDH3p-cbbM-CYC1tbla |
This study. |
| pMK-RQ |
nptII |
Life Technologies Europe BV. |
| pUD230 |
PGI1p-cbbQ2-TEF2tnptII |
Life Technologies Europe BV. |
| pUD231 |
PGK1p-cbbO2-ADH1tnptII |
Life Technologies Europe BV. |
| pUD232 |
TEF1p-groEL-ACT1tnptII |
Life Technologies Europe BV. |
| pUD233 |
TPI1p-groES-PGI1tnptII |
Life Technologies Europe BV. |
| pUDC075 |
URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;PGI1p-cbbQ2-TEF2t;PGK1p-cbbO2-ADH1t;TEF1p-groEL-ACT1t;TPI1p-groES-PGI1tbla |
This study. |
| pUDC099 |
URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;PGI1p-cbbQ2-TEF2t;PGK1p-cbbO2-ADH1tbla |
This study. |
| pUDC100 | URA3 CEN6 ARS4 TDH3p-cbbM-CYC1t;TEF1p-groEL-ACT1t;TPI1p-groES-PGI1tbla | This study. |