Table 1.
Histone H3 mutants exhibiting altered rapamycin sensitivity
| Histone H3 residue | Rapamycin phenotype | Nucleosome location | Source |
|---|---|---|---|
| A1S |
−1 |
Tail |
This study |
| T3A |
+1 |
Tail |
This study |
| T3D |
0 |
Tail |
This study |
| Q5A |
+2 |
Tail |
This study |
| Q5E |
+2 |
Tail |
This study |
| K14A |
−2 |
Tail |
[36] |
| K14R |
0 |
Tail |
This study |
| K14Q |
0 |
Tail |
This study |
| R17A |
−2 |
Tail |
This study |
| R17K |
−2 |
Tail |
This study |
| K18A |
−1 |
Tail |
[36] |
| K18R |
−2 |
Tail |
This study |
| K18Q |
0 |
Tail |
This study |
| K23A |
−1 |
Tail |
[36] |
| K23R |
0 |
Tail |
This study |
| K23Q |
0 |
Tail |
This study |
| S28A |
−2 |
Tail |
This study |
| S28D |
0 |
Tail |
This study |
| P30A |
−1 |
Tail |
This study |
| K37A |
Lethal |
Tail |
This study |
| K37R |
0 |
Tail |
This study |
| K37Q |
0 |
Tail |
This study |
| R49A |
−2 |
Lateral |
This study |
| R49K |
0 |
Lateral |
This study |
| K56A |
−3 |
Lateral |
[36] |
| K56R |
−2 |
Lateral |
[36] |
| K56Q |
+2 |
Lateral |
[36] |
| S57A |
+1 |
Disk |
[36] |
| S57D |
+1 |
Disk |
[36] |
| E73A |
−1 |
Disk |
This study |
| E73Q |
−1 |
Disk |
This study |
| Q76A |
−1 |
Disk |
This study |
| Q76E |
−1 |
Disk |
This study |
| T80A |
+1 |
Disk |
This study |
| T80D |
−1 |
Disk |
This study |
| L82A |
−1 |
Disk |
This study |
| R83A |
−2 |
Lateral |
This study |
| R83K |
0 |
Lateral |
This study |
| Q85A |
−2 |
Lateral |
This study |
| Q85E |
0 |
Lateral |
This study |
| S86A |
−2 |
Lateral |
This study |
| S86D |
−2 |
Lateral |
This study |
| L92A |
+1 |
Buried |
This study |
| E105A |
−1 |
Disk |
This study |
| A110S |
−1 |
Buried |
This study |
| A111S |
−1 |
Buried |
This study |
| A114S |
−1 |
Disk |
This study |
| V117A |
−3 |
Lateral |
This study |
| Q120A |
+1 |
Lateral |
This study |
| K121A |
−1 |
Disk |
This study |
| K121R |
0 |
Disk |
This study |
| K121Q |
−1 |
Disk |
This study |
| K122A |
−1 |
Disk |
This study |
| K122R |
−3 |
Disk |
This study |
| K122Q |
−1 |
Disk |
This study |
| R129A |
+1 |
Disk |
This study |
| R129K |
0 |
Disk |
This study |
|
Total number of H3 mutants screened |
114 |
||
|
Number of H3 mutants with phenotype |
30 |
||
|
Percentage of total H3 mutants with phenotype |
26.3% |
||
|
Percentage of H3 mutants with resistant phenotypes |
26.7% |
||
| Percentage of H3 mutants with sensitive phenotypes | 73.3% | ||