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. 2013 May 27;22(19):3858–3868. doi: 10.1093/hmg/ddt241

Figure 6.

Figure 6.

Recovery of cilia number following depolymerization (A). Control and cytochalasin D treated WT and Bbs4 null kidney primary cells. General view of the confluent field and representative cilia (inset). Treatment with cytochalasin D recovers cilia numbers and length in Bbs4 null cells (white arrowhead) compared with WT cells (white arrows). Scale bar 20 μm. Inset scale bar 10 μm. (B) Graphical representation of cilia length of treated and untreated WT and Bbs4 null cells. Untreated WT cells (2.55 μm ± 0.41), Treated WT cells (4.67 μm ± 0.53), untreated Bbs4 null cells (2.16 μm ± 0.23), treated Bbs4 null cells (7.58 μm ± 0.62). (C) Percentage of ciliated cells in relation to total number of cells. Untreated WT cells (48% ± 6.1), treated WT cells (51% ± 3.2), untreated Bbs4 null cells (23.01% ± 7.02), treated Bbs4 null cells (49.29% ± 3.9). Note that there is no increase in the percentage of WT ciliated cells following treatment with cytochalasin (D); however treated Bbs4 null cells recover to WT levels. ***P < 0.001, *P < 0.05, ns; not- significant.